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Ovarian responses after superovulation with different dosages of oFSH in goats

Abdullah¹*, R.B., Shariffah², N.Y., Rahman¹, M.R. and Wan Khadijah¹, W.E.

¹Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia,
²Department of Veterinary Services Malaysia, 62530 Putrajaya, Malaysia.

Malaysian J. Anim. Sci. 2012 15(1): 21-25

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A total of 35 crossbred goats (Capra hircus) were used to evaluate the effect of oFSH dosages on ovarian responses during superovulation. A controlled internal drug release (CIDR) was inserted for 14 days in each goat. The animals were divided into 3 groups. Groups M1 and M2 received 8.8 mg and 14.08 mg, respectively, of oFSH through multiple intramuscular (i.m.) injections starting from 2 days before the CIDR removal and Group S received 8.8 mg of oFSH through a single i.m. injection upon CIDR removal. Ovarian responses of all treatments were evaluated during laparotomy session on Day 3 or 7 after CIDR removal. All of the does (100%, 22/22) of Groups M1 and M2 responded positively to the treatments by ovulating at least 1 tertiary follicle. On the other hand, only 69% (9/13) of the does of Group S responded correspondingly to the treatment. The total ovarian responses (CL plus anovulatory follicles) among the treatments were significantly higher (P<0.05) in Groups M1 and M2 compared with Group S. There were no significant differences between multiple-administration groups for CL per doe (high dosages vs. low dosages: 16.9�3.9 vs. 11.6�1.0, respectively). The percentage of anovulatory follicles was significantly higher (P<0.05) in Group S followed by Groups M1 and M2. The structure (embryo plus oocyte) recovery rate per doe was significantly the highest (P<0.05) in Group M2 followed by Group M1 and Group S (5.4�2.4, 3.2�1.2 and 0.5�0.5, respectively). The numbers of embryos per doe were 0.1, 2.4 and 2.0, for Groups S, M1 and M2, respectively. It is concluded that multiple injection at high dosage (14.0 mg) of oFSH in goat superovulation protocol gives better structure recovery than multiple injection at low dosage (8.8 mg) as well as single injection (8.8 mg) of oFSH. However, more detailed research is needed to optimize the dosage of oFSH for viable embryo recovery during superovulation of Malaysian crossbred goats.