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Analysis of ovine uterus Expressed Sequence Tags (ESTs)

Suhaimi, A.H.M.S ¹*, I.A. Azlina Azma², A.R. Roziatul Erin¹, W. M. Z. Somarny¹, M. N. Mat Isa³, Z. A. Rabiatul Adawiah⁴ , S. Md Tasol¹ and K. Musaddin¹

¹Strategic Livestock Research Centre, MARDI Headquarters, 43400 Serdang, Selangor.
² MARDI Research Station Muadzam Shah, Muadzam Shah, Pahang.
³Malaysian Genome Institute, Bangi, Selangor.
⁴⁵ Biotechnology Research Centre, MARDI Headquarters, 43400 Serdang, Selangor

Malaysian J. Anim. Sci. 2012 15(1): 1-12

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The aim of this paper was to explore the different analyses available to examine Expressed Sequence Tags (ESTs). ESTs were generated from the uterus of indigenous sheep, Malin. A total of 675 unigenes were identified of which 603 were singletons and 72 were consensus sequences. Seventy nine of the unigenes had significant match to genes in SwissProt database while the remaining unigenes had to be converted to coding regions (CDS). Fifty seven of the CDS had significant matches to the SwissProt database. Upon functional annotation to both unigenes and CDS, binding and catalytic activity were the two most common molecular function while the most common biological processes were related to cellular processes. Enzyme commission pathway related to DNA, purine and primidine metabolism pathway had the most abundant number of genes Subjected to KEGG pathway mapping. Pfam protein family related to electron transport process towards the production of ATP family, was additionally identified among the ESTs. Analysis of ESTs with Pfam revealed the importance of ATP generation in the uterus. Albeit minimally, the different analyses performed in this project provide some light in the function of ovine uterus and can be applied to other indigenous species that have no or limited genome data availability to discover novel and unknown genes and proteins.