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Mannan extract from palm kernel cake to control Salmonella thypimurium in broiler chickens

Tafsin*, M., Hanafi,N.D., Nur'aini, N, and Yusraini, E.

Animal Science Study Program, Faculty of Agriculture, Universitas Sumatra Utara, Medan, North Sumatera 20155, Indonesia

Malaysian J. Anim. Sci. 2018 21(1): 73-83

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Palm kernel cake has a high content of mannan that has the potential to be used as a supplementary feed through an extracting process which functions to inhibit the colonization of bacteria that harm poultry. The purpose of this research was to examine the capability of mannan extract from palm kernel cake to control Salmonella thypimurium in broilers. The effect of mannan extract from palm kernel cake was done by agglutination, resistance and inhibitory tests in liquid medium. In vivo test was carried on 3-day old broilers which were orally infected with 105 CFU Salmonella thypimurium. Levels of mannan extract given were 0, 0.1, 0.2, 0.3 and 0.4%. The results showed that mannan extract from palm kernel cake had no bactericidal and bacteriostatic activity, but showed the agglutination ability when evaluated  microscopically.  The utilization of mannan extract from palm kernel cake at 0.3% and 0.4%   showed significantly (P<0.05) higher number of Salmonella thypmurium (cfu/g) on feaces compared to the control (7.00 and 6.91 vs 6.69, respectively).  Increasing level of  mannan extract significantly (P<0.05) decreased the total number of bacterial colonies in caecum and the numbers of Salmonella thypmurium (cfu/g) at the levels of 0, 0.1, 0.2, 0.3 and 0.4% were 4.56; 4.51; 4.37; 4.13; 3.62, respectively.  The utilization of mannan extract from palm kernel cake had no significant effect on caecal pH and the average daily gain of broiler chickens. It is concluded that mannan extract from palm kernel cake has the ability to reduce colony of Salmonella thypimurium in broilers so that it can be used as a feed supplement in broiler chickens diet.