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Identification of myostatin gene on Kacang and Peranakan Etawah goats in Deli Serdang District, North Sumatera, Indonesia

Hasyim, A.R.^1*, Tafsin, M.² and Batubara, A.³

¹Graduate Magister of Animal Science Program, Faculty of Agriculture, University of North Sumatera, Medan-Indonesia,
²Animal Science Program, Faculty of Agriculture, Universty of North Sumatera, Medan-Indonesia,
³Loka Research Center Kambing Potong Sei Putih, North Sumatera, Indonesian

Malaysian J. Anim. Sci. 2018 21(1): 1-10

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One of the important genes that affects the growth of goats is the myostatin(MSTN) gene. The purpose of this study was to detect the polymorphism of the MSTN gene and identify SNP in the fragment target MSTN gene exon 1 in Kacang and Peranakan Etawah goats in Deli Serdang District, Sumatra Indonesia. The number of samples used was 60 goats consisting of 30 Kacang goats and 30 Peranakan Etawah (PE) goats from Galang, Hamparan Perak and Namorambe, Deli Serdang district.  The MSTN gene forward primer was 5?-AGA ACA GCG AGC AGA AGG AA-3? and reverse primer 5?-CGT CGT AAC GTG GTA GTC AT-3?, the enzyme for restriction HinIII. To determine Hardy-Weinberg equilibrium status, both allele and genotype frequencies were analyzed. To align sequencing results  BioEdit and MEGA6 softwares were used. The results showed that there were MSTN gene exon 1 found and SNP in 53 points in Kacang and PE goat populations in the district sampled and was monomorphic. Only one genotype from MSTN exon 1 was discovered, that was AA. Heterozygosity and HW equilibrium  was 0, because  only one genotype was found in MSTN gene in this study. Myostatingene at Kacang and PE goat populations in Deli Serdang District is therefore found to be monomorphic.