MJAS

Manuscript Details

MtDNA diversity and genetic lineages of four cattle breeds in Malaysia

Somarny¹*, W.W.M.Z., Ruzainah², A., Aslinda³, K. Mohd. Hafiz⁴, A.W., Md Tasol¹, S., Fadzlirahimi⁵, I., Amie Marini¹, A.B. and Siti Azizah³, M. N.

¹Animal Science Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang, Selangor.
²Bio-Engineering Section, University Kuala Lumpur ? Institute of Chemical and Bio-Engineering Technology (UniKL-MICET), Alor Gajah, Melaka.
³Aquaculture Research Group, School of Biological Sciences, Universiti Sains Malaysia, Minden, Penang.
⁴Muadzam Shah MARDI, Pahang.
⁵Kemaman MARDI, Terengganu.

Malaysian J. Anim. Sci. 2015 18(1): 23-33

Click to view the abstract

There is lack of comprehensive studies on the genetic diversity or phylogenetic analysis of beef cattle breeds in Malaysia. In this study, the partial sequence of mitochondrial DNA cytochrome b gene (cyt b) was analysed from blood samples obtained from 25 Chinese Yellow Cattle (CY), 33 Kedah-Kelantan (KK), 32 Brakmas (BM) and 30 Bali cattle (BC). Based on these 120 individuals, 19 mtDNA haplotypes (GenBank Accession No. GU67340 - GU67358) were identified by polymorphisms at 31 sites. Hap19 was predominant in BM (78%), KK (82%) and CY (100%) indicating similar origin or gene flow between breeds whilst Hap11 was exclusively for BC. However, there were only two nucleotide differences between these two major haplotypes. These results can be interpreted that these representative cattle in these haplotypes are admixtures of B. indicus or B. javanicus through maternal ancestry. Conversely, the CY cattle investigated are highly inbred where no variation could be observed in the short segment investigated.